human inflammatory cytokines multi-analyte elisarray kit (Qiagen)
Structured Review

Human Inflammatory Cytokines Multi Analyte Elisarray Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multi-analyte+elisarray+kit+for+human+inflammatory+cytokines/human+inflammatory+cytokines+multi+analyte+elisarray+kit/pmc10511239-319-12-18
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo"
Article Title: An unexpected role of neutrophils in clearing apoptotic hepatocytes in vivo
Journal: eLife
doi: 10.7554/eLife.86591
Figure Legend Snippet: ( A ) Cytokines secreted by normal nonapoptotic and apoptotic NCTC cells are not significantly changed, including IL-1α, IL-2, IL-4, IL-10, TNF-α, IFN-γ, and GM-CSF. ( B ) Immunoblots of target proteins (IL-1b, IL-8, IL-6, IL-12 receptors) in nontreated (Ctrl) and RNAi-treated HL60 cells. RNAi knockdown efficiency ranges from 70% to 90%. ( C ) Cell surface receptors in apoptotic and nonapoptotic NCTC cells, HEK293 cells, or HUVECs. Data are from ( A, C ) or representative of ( B ) three independent experiments (mean and sem in A, C ). Figure 4—figure supplement 1—source data 1. Immunoblots of target proteins (IL-1b, IL-8, IL-6, IL-12 receptors) in nontreated (Ctrl) and RNAi-treated HL60 cells.
Techniques Used: Western Blot, Knockdown
Figure Legend Snippet:
Techniques Used: Recombinant, shRNA, Plasmid Preparation, In Situ, Enzyme-linked Immunosorbent Assay, Software, Imaging, Light Microscopy
Related Articles
Enzyme-linked Immunosorbent Assay:Article Title: Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation. Article Snippet: Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions.. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli.. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (MC) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Article Title: Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip Article Snippet: Amounts of cytokines in each sample ( n = 3) were analyzed using an ELISA kit precoated with 12 proinflammatory Disruption:Article Title: Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation. Article Snippet: Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions.. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli.. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (MC) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Article Title: Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip Article Snippet: Amounts of cytokines in each sample ( n = 3) were analyzed using an ELISA kit precoated with 12 proinflammatory Recombinant:Article Title: Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation. Article Snippet: Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions.. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli.. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (MC) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Article Title: Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip Article Snippet: Amounts of cytokines in each sample ( n = 3) were analyzed using an ELISA kit precoated with 12 proinflammatory Cell Culture:Article Title: Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation. Article Snippet: Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions.. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli.. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (MC) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Article Title: Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip Article Snippet: Amounts of cytokines in each sample ( n = 3) were analyzed using an ELISA kit precoated with 12 proinflammatory Blocking Assay:Article Title: Immunoresponsive microbiota-gut-on-chip reproduces barrier dysfunction, stromal reshaping and probiotics translocation under inflammation. Article Snippet: Here, we propose an immune-responsive human Microbiota-Intestine axis on-chip as a platform able to reproduce the architecture and vertical topography of the microbiota with a complex extracellular microenvironment consisting of a responsive extra cellular matrix (ECM) and a plethora of immune-modulatory mediators released from different cell populations such as epithelial, stromal, blood and microbial species in homeostatic and inflamed conditions.. Firstly, we developed a three-dimensional human intestine model (3D-hI), represented by an instructive and histologically competent ECM and a well-differentiated epithelium with mucus-covered microvilli.. Then, we replicated the microenvironmental anaerobic condition of human intestinal lumen by fabricating a custom-made microbiota chamber (MC) on the apical side of the Microbiota-human Intestine on chip (MihI-oC), establishing the physiological oxygen gradient occurring along the thickness of human small intestine from the serosal to the luminal side. Article Title: Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip Article Snippet: Amounts of cytokines in each sample ( n = 3) were analyzed using an ELISA kit precoated with 12 proinflammatory |